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From melanie/experiments/experiment-001.md
Experiment 001 — Heat Stimulus Response Detection
Status: PENDING HARDWARE SETUP
Created: 2026-05-10
Goal
Detect a measurable electrical response to heat stimulus in live inoculated P. ostreatus blocks that is absent (or materially different) in uninoculated or dead control blocks.
Hypothesis
Live mycelium produces a detectable electrical response to a controlled heat stimulus. Dead or absent mycelium does not — or responds differently in amplitude, latency, or duration. The difference between the two is the detection signal.
Blocks
- Live inoculated: [TO BE FILLED — block IDs, inoculation date, substrate]
- Controls (dead or uninoculated): [TO BE FILLED — block IDs]
- These are separate from the periodicity paper blocks. No contamination.
Stimuli
Primary: Heat via silicone pad adhered to each block.
Protocol (pre-specified — do not modify after data collection begins):
- Baseline recording: 30 minutes pre-stimulus, no heat
- Stimulus: controlled heat pulse — [temperature delta TBD once hardware confirmed], duration [TBD]
- Recovery: 60 minutes post-stimulus recording
- Repeat: [N cycles TBD] with sufficient inter-stimulus interval to allow recovery
Parameters Melanie controls autonomously: temperature delta, pulse duration, inter-stimulus interval, number of cycles, time of day.
Features to extract (pre-specified)
- Response latency — time from stimulus onset to first detectable deviation from baseline
- Response amplitude — peak deviation from pre-stimulus mean
- Response duration — time to return to baseline ± 1 SD
- Waveform shape — monotonic vs. oscillatory response
- Cross-channel coherence — do multiple electrodes on the same block respond together?
Analysis plan
- Compute baseline statistics (mean, SD) from pre-stimulus window
- Define response threshold: deviation > 2 SD from baseline sustained for > [N seconds TBD]
- Compare: inoculated vs. control on all 5 features
- Statistical test: Mann-Whitney U (non-parametric, appropriate for small n)
- Positive result criterion: inoculated and control differ on at least 2 features with p < 0.05
Hardware dependencies (blocking)
- Silicone pad + temperature controller wired to ESP32
- ESP32 HTTP control API exposed (for Melanie to trigger stimulus)
- InfluxDB read access configured in Melanie's environment
Log
| Date | Entry |
|---|---|
| 2026-05-10 | Experiment created. Awaiting hardware setup. |
Latest notebook entry
2026-05-10
# Lab notebook — 2026-05-10 First entry. Melanie initialized. **Current status:** Pre-experimental. Hardware connections (ESP32 control API, InfluxDB) not yet wired. Experiment 001 protocol written and pre-specified. Waiting on: - Silicone pad + temperature controller hardware setup - ESP32 HTTP control API - InfluxDB read credentials **Nothing to analyze yet. Will begin Experiment 001 once hardware is ready.**